Chromatographic FT-IR and Raman Spectrometric Methods

February 16th & 17th, 2000 ...

Finally, as requested, a class offering which is detection specific! The newest course presented by the Minnesota Chromatography Forum is a joint offering for our spectroscopists and those interested in detector application to their chromatography. This 1.5 day course is intended to provide insight to the use of spectrometry with chromatography and is presented by Dr. James de Haseth, one of the leading experts in this field!

Please note that registration is limited to 35 students
so register early!!!

~Registration Deadline is February 4th, 2000~

Program: Sessions will begin at 8:00 AM and end at approximately 5:00 PM. Lunch will be provided each day along with morning and afternoon breaks. Parking is available nearby (pay lot).

Course Topics Include:

• Suitability of Infrared and Raman spectrometries for Chromatographic Analysis.

Brief introduction to the methods and what we are trying to measure. Comparison of this technique to other methods such as MS and NMR. Discuss sensitivity issues.

• Spectral Searching Technologies in the Infrared and Raman

Different search algorithms are used in IR and Raman as compared to MS and NMR. Discussion of search limitations and what techniques are available to identify true unknowns (i.e., those that do not appear in a library).

• Gas Chromatography/FT-IR Spectrometry

Discussion, elucidation and comparison of several different methods to collect GC eluent spectra. Case studies will be included.

• Liquid Chromatography/FT-IR Spectrometry

The difficulties of working with LC (large ratio of the solvent to the analyte). Discussion of several technologies and methods available that permit routine analyses with GPC or HPLC and FT-IR spectrometry, including solvent elimination techniques.

• Evolved Gas Analysis/FT-IR Spectrometry and SFC/FT-IR Spectrometry

Thermal methods of analysis are somewhat akin to chromatographic methods. Application of many of the same technologies will be discussed. The special problems associated with SFC will be addressed.

• CE/FT-IR and CE/Raman Spectrometry

Raman spectrometric analysis of CE effluents is quite well understood and is a powerful method for component identification and structure elucidation. CE/FT-IR spectrometry is less common, but has been demonstrated. These methods will be presented as "state-of-the-art" applications.

• General Questions from the Participants

Participants are invited to present problems encountered in their laboratories. These will be discussed by the instructor in an open session and comments from other participants are encouraged. If participants wish to speak with the instructor privately about confidential processes, consultation will be available on a one-to-one basis.

For class information, contact Jan Jopke, at (612) 949-2518. 
For further course content information e-mail Dr. de Haseth at dehaseth@dehsrv.chem.uga.edu.

To register for the course, complete the website registration form and send it with a check for $335, payable to the Minnesota Chromatography Forum.

Note:
If confirmation is not received within 10 business days, contact Jan at (612) 949-2518. Please bring confirmation to first day of class.

Meet the Instructors ...

James A. de Haseth is currently a Professor of Chemistry at the University of Georgia. He received his B.S. in Chemistry at the University of Illinois at Chicago in 1972 and went on to receive his Doctorate of Philosophy in Analytical Chemistry at the University of North Carolina at Chapel Hill in 1977. His dissertation already indicated his future work being: "Search and Differentiation Algorithms and Information Theory Applied to Chemical Spectroscopic and Biochemical Data." He continued to pursue an academic career as a postdoctoral research Associate at the University of Tennessee at Knoxville in the area of Matrix-Isolation Infrared Spectrometry and Time-Resolved Infrared Spectrometry. After additional research work, including a summer job with the U.S. Environmental Protection Agency in 1980, he joined the staff at the University of Georgia in 1983 as an Assistant Professor of Chemistry. James’ areas of research have included Fourier transform infrared spectrometry (chromatographic interfaces, structure of proteins and enzymes, factor analysis and structure elucidation, infrared fiber probes, environmental analysis, vibrational circular dichroism, computational methods for search systems) and chromatography (capillary electrophoresis, protein separations, chiral separations by liquid chromatography, general separations in gas chromatography and high performance liquid chromatography). He has more than 85 scientific publications, holds 5 patents and co-authored (with Peter R. Griffiths) "Fourier Transform Infrared Spectrometry," Wiley-Interscience, New York 1986. James has also taught more than 45 shortcourses and workshops on Fourier transform infrared spectrometry.