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Winter Quarterly Meeting
Proteins, Peptides, and
Proteomics
by LeeAnn Higgins,
Ph. D.
Department of Biochemistry,
University of Minnesota
| Date: |
March 4, 2003
6 pm Social Hour
7 pm Presentation |
| Location: |
Braun Intertec Corporation
11001 Hampshire Ave S.
Bloomington, MN
(Directions below) |
Abstract:
Practical considerations of LC/MS,
using 1 and 2 dimensional HPLC, as well as limitations of the technique and
biologically reasonable goals and expectations will be discussed in the
context of current projects
The Speaker
Ms. Higgins works at the Mass Spectrometry Consortium for the Life
Sciences which is a core facility at the University of Minnesota that
supports researchers achieve milestones and test hypotheses using mass
spectrometry (MS). This is often done in conjunction with
separations methods, such as HPLC and GC. Mass spectrometry provides
structural information for unknown compounds such as proteins,
peptides and small molecules. MS combined with a chromtographic technique
results in a methods by which complex mixtures of unknowns can be
identified . Separation (by capillary HPLC), identification (by MS)
and relative quanitification of proteins (using ICAT reagents) is a common
goal and is now practical with the state-of-the-art instrumentation at the
MSCLS.
Educational background
B.S., Medical Technology, State University of New York at Albany, Albany,
NY
M.S., Clinical Chemistry, Rochester Institute of Technology, Rochester, NY
Ph.D., Pharmacology, University of Rochester, Rochester, NY
Postdoctoral, Pathogenesis, Univeristy of Washington, Seattle, WA
Postdoctoral, Proteomics, University of Minnesota, St. Paul, MN
Professional Background
Currently, Ms. Higgins is a Research Assistant Professor, UM,
Department of Biochemistry, Molecular Biology & Biophysics. Her
current project is Mass spectrometric analysis of proteins and small
molecules at femtomole and picomole levels in a project-oriented mass
spectrometry facility. Teaching and assisting with mass
spectrometric approaches to proteomics in the Biological, Chemical,
Agricultural, Medical and Pharmaceutical Sciences. She also held
positions of Research Associate and Postdoctoral Associate at the U of M.
While serving as a Postdoctoral Research
Associate, at the University of Washington, Seattle, WA, she worked
on: Mass spectrometry and capillary HPLC; Protein sequencing and
characterization of the bacterial toxins phospholipase C-H and PLC-R from
Pseudomonas aeruginosa.
Directions:
Braun Intertec Corporation
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